diff --git a/pytest_tests/testsuites/services/s3_gate/test_s3_bucket.py b/pytest_tests/testsuites/services/s3_gate/test_s3_bucket.py
index 0824f44a..16402e77 100644
--- a/pytest_tests/testsuites/services/s3_gate/test_s3_bucket.py
+++ b/pytest_tests/testsuites/services/s3_gate/test_s3_bucket.py
@@ -140,3 +140,30 @@ class TestS3GateBucket:
             s3_client.delete_bucket(bucket)
             with pytest.raises(Exception, match=r".*Not Found.*"):
                 s3_client.head_bucket(bucket)
+
+    @allure.title("[NEGATIVE] Delete non-empty bucket (s3_client={s3_client})")
+    def test_s3_check_availability_non_empty_bucket_after_deleting(
+        self,
+        bucket: str,
+        simple_object_size: ObjectSize,
+        s3_client: S3ClientWrapper,
+    ):
+        object_path = generate_file(simple_object_size.value)
+        object_name = s3_helper.object_key_from_file_path(object_path)
+
+        with reporter.step("Put object into bucket"):
+            s3_client.put_object(bucket, object_path)
+
+        with reporter.step("Check that object appears in bucket"):
+            objects = s3_client.list_objects(bucket)
+            assert objects, f"Expected bucket with object, got empty {objects}"
+            assert object_name in objects, f"Object {object_name} not found in bucket object list {objects}"
+
+        with reporter.step("Try to delete not empty bucket and get error"):
+            with pytest.raises(Exception, match=r".*The bucket you tried to delete is not empty.*"):
+                s3_client.delete_bucket(bucket)
+
+        with reporter.step("Check bucket availability"):
+            objects = s3_client.list_objects(bucket)
+            assert objects, f"Expected bucket with object, got empty {objects}"
+            assert object_name in objects, f"Object {object_name} not found in bucket object list {objects}"